The paper describes the creation of a regional innovation system, which is one of the mechanisms of technological entrepreneurship, which is especially important for the Russian Far East. It is shown that traditional views on the formation of regional innovation systems are revised in modern conditions. This process occurs most productively within the triple helix model. The study describes the triple helix model, examines the motivations of the actors for implementing innovations and networking. It is concluded that regional factors influence the intensity and forms of involving universities, enterprises and the state in these processes, in particular using channels for the transfer of knowledge and technology. We have formulated the hypothesis that technological entrepreneurship in the region could achieve more effective development by establishing the mechanism of interaction of all elements of the triple helix, that is, the university, the state and the business. We have justified the special role of the technical university in the development of innovative entrepreneurship in the Komsomolsk Territory of Advanced Development (TAD). It is caused by three types of interaction with the enterprises of the innovation cluster in Khabarovsk Krai: the implementation of educational programmes in priority areas of training, retraining and improvement of professional skills of the staff; conducting applied scientific research jointly with business; sharing innovative infrastructure of the university. It has been proved that networking between the participants of the educational process when using innovative infrastructure allows to build a continuous chain of transforming scientific knowledge into new or improved products or technologies in the region. The goal of the study is to substantiate targeted development of technological entrepreneurship in the region on the basis of the triple helix model. The authors analysed the network communication in the process of exchange of knowledge (transfer) between objects of the innovation infrastructure of the university and the region. We have revealed the trajectory of innovation-specific ‘education-science-technology-business’ chains in the directions of the national technological initiative. We have proposed recommendations for improving the transfer of knowledge, the level of innovative activity of economic entities, sensitivity to knowledge and technology at the local level. The results of this study can be used by the authorities and participants in networking in order to form a triple helix model.